Journal: Heliyon
Article Title: Tumor microenvironment (Part I): Tissue integrity in a rat model of peripheral neural cancer
doi: 10.1016/j.heliyon.2024.e33932
Figure Lengend Snippet: Schematic representation of the experimental design. AT-1 Cells were injected within the SNs' perineurial sheath of Copenhagen rats. Different groups were recruited for each timepoint. A sham-operated group was also recruited, in all steps, for comparison purposes. Three paths were taken to perform these experiments. The morphological changes of the extracted SNs were photographed ( 1 ). Tissues of the SNs were sliced, stained by H&E, and visualized under light microscope ( 2 ). For the third path, SN-tissues have been taken away using a different protocol, incubated with the primary and then the fluorochrome-tagged-secondary antibody. Fluorescence captured by confocal laser microscope has been further processed, and the measured intensities were statistically analyzed ( 3 ).
Article Snippet: The prepared slides were histologically visualized by means of hematoxylin and eosin (H&E) and an inverted light microscope (Axiovert 25, Carl Zeiss, Germany) assembled with a cooled charge-coupled device (CCD) camera (AxioCam HRc).
Techniques: Injection, Comparison, Staining, Light Microscopy, Incubation, Fluorescence, Microscopy